October 2007

Studies by Dr Xiaoyang Qi at Cincinnati Children’s Hospital (Figure1) show that membranes labeled with CellVue Maroon can be imaged using Xenogen’s IVIS imaging system.

	A subcutaneous tumor was established on the right shoulder of mouse (1) as indicated by the black circle. Mouse (2) is a non-tumor bearing control. After 36 days, tumor-bearing and non-tumor control mice were injected via tail vein with tumor-targeted protein-lipid nanovesicles labeled with CellVue Maroon. Mice were imaged 24h post injectin using the IVIS 200X imaging system (Xenogen, Inc) with scanning time 0.1 sec and Cy5 filter set. A strong fluorescent signal was seen at the tumor site (mouse 1).
Images courtesy of Dr. Xiaoyang Qi (Cincinnati Children's Hospital and medical Center) and Bexion Pharmaceuticals.

Studies by Dr David Anderson at Burnet Institute (see movie on right) and Dr Edward Roy at the University of Illinois (Figure 2) show that cell membranes labeled with CellVue Burgundy and CellVue NIR-815 are well matched for imaging studies using LI-COR Biosciences Odyssey Imaging System and can be detected with minimal signal overlap.



Figure 2: Mouse lymphocytes were stained with either 10 µM CellVue® Burgundy or CellVue NIR815 for 5 min at 37oC then washed 5x with media according to the Molecular Targeting Technologies, Inc. protocol. They were then spotted on a slide, alone and together and imaged using the 700 and 800nm channels of the Odyssey Infrared Imaging System (LI-COR Biosciences, NE). The left side image shows the lymphoctes stained with CellVue Burgundy (Red), the middle image shows the mixture of both CellVue Burgundy and CellVue NIR815 labelled cells and the right side image shows the lymphoctes labelled with CellVue NIR815 (Green). Note that since the Burgundy labeled cells were 10x as concentrated as the NIR815 labeled ones they appear as a larger population. Images courtesy of Dr Edward Roy, University of Illinois.

A monocyte cell line (U937) was labeled with CellVue Burgundy (red) and a T-Cell Line (SUPT1) was labeled with CellVue NIR815 (green) according to the Molecular Targeting Technologies, Inc. protocol. A million monocytes and T-cells were spotted separately with some overlap on to a glass fiber pad and imaged using the Odyssey Infrared Imag-ing System (LI-COR Biosciences, NE). The 3 images display the same sample pad viewed through different filters. Image A was taken using the 700nm emission filter and shows only monocytes, Image B was taken using the 800nm emission filter and shows only T-cells and Image C was taken using combined 700 and 800nm filters and shows both cell populations clearly visible.
Images courtesy of Simone van de Waarsenburg and Dr David Anderson, Burnet Institute, Australia