PSVue reagents are a family of fluorescent probes containing
a bis(zinc2+dipicolylamine) group (Zn-DPA), a motif that has been
found to bind with high affinity to surfaces enriched with anionic phospholipids,
especially phosphatidylserine (PS) exposed on cell membranes.
The fluorescent part of the probe is a reporter element that
provides a means of detecting the probe once it is bound to the membrane
Key Features of PSVue® Probes:
- Bind to a variety of cell types which have negatively charged
phospholipids exposed on their membranes including apoptotic
cells , necrotic cells , Gram+ and Gram- bacteria [3-
5], activated cells, tumor vascular endothelial cells, viruses, etc.
- Available in a range of detection wavelengths from long-UV to
- Suitable for in vitro and in vivo use.
- Suitable for high-throughput screening assays.
- Bind to the same PS site as annexin-V.
Advantages of PSVue® over Fluorescent Annexin-V:
- Binding kinetics are fast; annexin-V binding is slow
- Binding is Ca2+ independent; means no artifacts due to activation
of nonspecific membrane scramblases by Ca2+.
- Cheap compared to most annexin-V fluorescent analogs
- Apoptosis can be detected under a wide variety of conditions
(e.g. in presence of 10% serum, temps from 4 to 37oC).
- Can provide more intense labeling due to their much smaller
size (i.e. >10 PSVueTM molecules can bind to the same area as
1 annexin V molecule).