NeuroVue® Dye Filters for NEURONAL TRACT TRACING

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Professor Fritzsch Shows Six Color Neuronal Tract Tracing

Advantages Include:

Convenient, ready-to-use coated filter format
More precise control of dye insertion point
No messy oils, pastes or hard-to-position crystals
Diffusion properties comparable to or better than other commercially available neurotracing dyes
More focal results (e.g. labeling of small sets of axons within a pathway)
Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression

Recent Publications

Meeting Presentations:

Diffusion properties of lipophilic dyes within and between adjacent cell membranes[pdf]. Fritzsch B, Muirhead KA, Gray BD, Feng F and Ohlsson-Wilhelm BM Presented at ISAC XXIII, Quebec City, Canada, May 22, 2006.
Analysis of Multicolor Neuronal Profiling using Novel Lipophilic Dyes [pdf] . Jensen-Smith H, Gray B, Muirhead K, Ohlsson-Wilhelm B and Fritzsch B. Presented at the Association for Research in Otolaryngology meeting, February 16-21, 2008, in Pheonix, Arizona
Photo- and bio-physical characterization of novel blue and near-infrared lipophilic fluorophores for neuronal tracing [pdf]. Tonniges J, Hansen M, Fritzsch B, Gray B and Nichols MG. Presented at the American Physical Society meeting, March 10-14, 2008, in New Orleans, Louisiana.

Protocols:

NT001: General protocol for the use of NeuroVue filters in fixed tissue[pdf].

NeuroVue Dye Solid:

NeuroVue Red, Maroon and Jade are also available in solid form for researchers who would like to make their own dye-coated materials for application to tissue (e.g. use for coating glass/metal pins or pieces of hair to provide a more rigid support when smaller injections are required).

Images 1-3 show that the diffusion properties of NeuroVue (NV) Maroon and NV Red are well matched. Filter strips were inserted into the olivocochlear efferent bundle in the brainstem near the facial genu of a mouse and after 4 weeks at 36oC the cochlea was prepared as a flat mount and imaged. Both dyes exhibit comparable diffusion from the brainstem to the cochlea (Images 1 and 2) and the near identical overlap of both dyes (Image 3) suggests almost identical lateral and transcellular diffusion properties.

Images 4-6 show that the diffusion properties of NV Maroon and NV Orange are well matched. Filter strips were inserted into the olivocochlear efferent bundle in the brainstem near the facial genu of a mouse and after 2 weeks at 36oC the cochlea was prepared as a flat mount and imaged. Both dyes exhibit comparable diffusion from the brainstem to the cochlea (Images 4 and 5) and the near identical overlap of both dyes (Image 6) suggests nearly identical lateral and transcellular diffusion properties. The somewhat stronger labeling of NV Orange in more distant fibers indicates that this dye diffuses slightly more effectively.

Images 7-9 show the effects of tail length modification on transcellular diffusion. Filter strips co-coated with NV Red and a shorter tail analog of NV Maroon (PTIR284) were inserted into the olivocochlear efferent bundle in the brainstem near the facial genu of a mouse. After 2 weeks at 36 oC the cochlea was prepared as flat mount and imaged. As before for this diffusion time, NV Red labeled fibers are found around inner hair cells and some fibers extent to outer hair cells (Image 7). In contrast the green false colored shorter tail analog of NV Maroon (PTIR284) shows dramatic transcellular diffusion of almost all cells in the cochlea (green circles, black holes are nuclei, Image 8). The superposition of both dyes (Image 9) shows how further the shorter tail NV Maroon analog (green) has spread out compared to the longer tail NV Red (red). These results show that transcellular diffusion properties are strongly dependent on alkyl ‘tail’ number and length and suggest that transcellular diffusion maybe helpful in studying contacts in difficult to access tissue.

Images courtesy of Dr Bernd Fritzsch, Creighton University, Nebraska, USA.

Catalog Number	Name	Description	Applications	Price
FS-1001	NeuroVue® Maroon product insert	1cm2 filter coated with the lipophilic far red emitting dye, NeuroVue® Maroon (ex max=647nm; em max=667nm).	Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with most fluorescent genetic tags, and NeuroVue Red, Orange and Jade.	$188.00
FS-1002	NeuroVue® Red product insert	1cm2 filter coated with the lipophilic red emitting dye, NeuroVue® Red (ex max=567nm; em max=588nm).	Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Orange.	$188.00
FS-1003	NeuroVue® Orange product insert	1cm2 filter coated with the lipophilic reddish orange emitting dye, NeuroVue® Orange (ex max=550nm; em max=570nm).	Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP and YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Red.	$188.00
FS-1004	NeuroVue® Emerald	DISCONTINUED Replaced by NeuroVue® Jade, FS-1006
FS-1005	NeuroVue® Burgundy product insert	1cm2 filter coated with lipophilic far red emitting dye, NeuroVue® Burgundy (ex max =683nm; em max=707nm).	Useful for tract tracing studies of up to 7 days. Spectrally compatible with most fluorescent genetic tags and NeuroVue Red, Orange, Jade. Spectral unmixing required for use with NeuroVue Maroon.	$188.00
FS-1006	NeuroVue® Jade product insert	1cm2 filter coated with the lipophilic green emitting dye, NeuroVue Jade (ex max=478nm; em max=508nm	Useful for tract studies of up to 5 days. Spectrally compatible with NeuroVue Maroon, Orange and Red.	$188.00
FS-1007	NeuroVue® Red Plus product insert	1cm2 filter coated with the lipophilic red emitting dye, NeuroVue Red (ex max=567nm; em max=588nm. Dye loading on this product is ~1.5X that of FS-1002	Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Orange. Provides faster and more extensive labeling than FS-1002 in many cases.	$205.00
DY-1001	NeuroVue® Red Solid product insert	1 mg of solid dye	Can be formulated as desired for application.	$171.00
DY-1002	NeuroVue® Maroon Solid product insert	1 mg of solid dye	Can be formulated as desired for application.	$171.00
DY-1003	NeuroVue® Jade Solid product insert	1 mg of solid dye	Can be formulated as desired for application.	$171.00

NeuroVue® is a trademark of PTI Research, Inc. used under license.
NeuroVue® Products are sold under sublicense from PTI Research, Inc.
US Patent Numbers 7,462,347 B2 and 8,029,767 B2.